Exhibit 23.1

Independent Registered Public Accounting Firm’s Consent

We consent to the incorporation by reference in this Registration Statement of Alliqua BioMedical, Inc. on Amendment No. 1 to Form S-3 (File No. 333-197844) of our report dated March 24, 2014, with respect to our audits of the consolidated financial statements of Alliqua BioMedical, Inc. and Subsidiaries as of December 31, 2013 and 2012 and for the years then ended, appearing in the Annual Report on Form 10-K of Alliqua BioMedical, Inc. for the year ended December 31, 2013. We also consent to the reference to our Firm under the heading “Experts” in such Prospectus.

/s/ Marcum llp

Marcum llp

New York, NY

September 8, 2014